TheeffectsofautophagyonvascularendothelialcellsinducedbyairbornePM2.5

摘要： ThepurposeofthisstudywastoexaminethedirecttoxicityofPM2.5collectedfromBeijingonhumanumbilicalveinendothelialcells(HUVEC).ACellCountingKit8(CCK8)assaydemonstratedthatPM2.5exposuredecreasedtheproliferationofHUVECsinadosedependentmanner.WealsofoundthatPM2.5exposureinducedautophagyinHUVECs,asevidencedby:(1)anincreasednumberofdouble-membranevesicles;(2)enhancedconversionandpunctuationofthemicrotubule-associatedproteinlightchain3(LC3);and(3)decreasedlevelsoftheselectiveautophagysubstratep62inatime-dependentmanner.Furthermore,promotingautophagyinPM2.5-exposedHUVECswithrapamycinincreasedthecellsurvivalrate,whereasinhibitingautophagyvia3-methyladeninesignificantlydecreasedcellsurvival.TheseresultsdemonstratethatPM2.5exposurecaninducecytotoxicityandautophagyinHUVECsandthatautophagyplayaprotectiveroleagainstPM2.5-inducedcytotoxicity.ThefindingsofthepresentstudyimplyadirecttoxiceffectofPM2.5onHUVECsandprovidenovelinsightintothemechanismofcardiovasculardiseasescausedbyPM2.5exposure. ... （共6頁）